Then at the 6 point will lead to a higher pregnancy rate. From the seperation result, the correct folding ratio of IL-2 molecules can be calculated. 据此可以计算出IL-2分子的正确折叠率。
A common method for refolding of recombinant proteins is the dilution method. With this method, the correct folding ratio will decrease when the refolding protein concentration increases. 常用的稀释复性方法,随着IL-2浓度的增高,它的正确折叠率逐渐降低,蛋白浓度的对数与正确折叠率之间大致呈线性关系。
For example, the correct folding ratio was only 30% when IL-2 concentration reached 1mg/ mL. But if refolding was carried out under very low protein concentration, the large sample volume will be difficult to handle. 当IL-2浓度为1mg/mL时,其正确折叠率仅为30%,而采取较低的蛋白浓度进行复性会因大量的样品体积导致后期纯化的困难。